Non-invasive biomarkers of disease progression in mice with cancer are lacking, making it challenging to implement appropriate humane end points. The authors conclude that temperature, food and water consumption were useful biomarkers of disease progression in mice with lymphomas and could potentially be used more widely to monitor mice with other forms of cancer.
The authors propose to modify current research policies. In particular they propose to discourage the common use of exploratory studies to support confirmatory inferences, but to 1) promote a greater volume of confirmatory investigations, and 2) to customize design and reporting guidelines for each mode.
The article focuses on the possibilities of reducing the number of animals by skipping small animal models, by evidence based selection of animal models and by choosing characteristics that increase their predictive value.
Vasectomy is a surgical procedure (severity level of 2) which until now is indispensable for the preparation of males which are subse‐ quently used to induce pseudopregnancy in foster females. Such females are required for strain rederivation by embryo transfer (ET) as well as other techniques that involve ET such as the production of transgenic and “knock‐ out” mice. We have recently refined all ET‐ dependant methods by generating a transgenic mouse strain carrying a dominant male‐specific sterility trait. Males from such a strain could be used directly to induce pseudopregnancy in females thus making the vasectomy procedure redundant. …
According to a new ordinance of the Federal Veterinary Office (BVET) issued May 1st, 2010, marking and biopsy taking of mice will have to be combined. Apart from ear punches, a method of choice is the clipping of toe tips of newborn mice. However, the method is dis‐ cussed controversially from an animal welfare point of view, both by authorities and the public.