Posts & Articles - Author: kbu

Using body temperature, food and water consumption as biomarkers of disease progression in mice with Eµ-myc lymphoma

Non-invasive biomarkers of disease progression in mice with cancer are lacking, making it challenging to implement appropriate humane end points. The authors conclude that temperature, food and water consumption were useful biomarkers of disease progression in mice with lymphomas and could potentially be used more widely to monitor mice with other forms of cancer.

Generation of transgenic mice: Replacement of vasectomized males by genetically sterile males

Vasectomy is a surgical procedure (severity level of 2) which until now is indispensable for the preparation of males which are subse‐ quently used to induce pseudopregnancy in foster females. Such females are required for strain rederivation by embryo transfer (ET) as well as other techniques that involve ET such as the production of transgenic and “knock‐ out” mice. We have recently refined all ET‐ dependant methods by generating a transgenic mouse strain carrying a dominant male‐specific sterility trait. Males from such a strain could be used directly to induce pseudopregnancy in females thus making the vasectomy procedure redundant. …

Marking transgenic mice by toe clipping of newborns

According to a new ordinance of the Federal Veterinary Office (BVET) issued May 1st, 2010, marking and biopsy taking of mice will have to be combined. Apart from ear punches, a method of choice is the clipping of toe tips of newborn mice. However, the method is dis‐ cussed controversially from an animal welfare point of view, both by authorities and the public.