Blog - Category: Transgenic Techniques

Generation of transgenic mice: Replacement of vasectomized males by genetically sterile males

Vasectomy is a surgical procedure (severity level of 2) which until now is indispensable for the preparation of males which are subse‐ quently used to induce pseudopregnancy in foster females. Such females are required for strain rederivation by embryo transfer (ET) as well as other techniques that involve ET such as the production of transgenic and “knock‐ out” mice. We have recently refined all ET‐ dependant methods by generating a transgenic mouse strain carrying a dominant male‐specific sterility trait. Males from such a strain could be used directly to induce pseudopregnancy in females thus making the vasectomy procedure redundant. …