Intravenous injection in black mice is difficult because the lateral tail vein used faintly contrasts to the skin. Here, an improved injection protocol is presented. It makes tail vein injection in black mice much more reliable and therefore represents a refinement of the method. The protocol describes the combined use of a special 180° LED illumination, of a Broome style restrainer as well as of effortless insulin syringes.
This article was kindly donated by Jürg Messer, Roche Pharma Research, Basel.